Clone

Target

IDP05156  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Oct 20, 2010  

Experiment End Date

Nov 08, 2010  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

344  

Reference Sequence Used

MIKKKYMNAF VIAATLAVPF SSIMAPIAKA EAAVEMKAAS KLADGTYDVI LKTYKDKTND TSVASTYLKN LKVTIQGDKK IVTLTVQDSS YFQYLRVEDT NKVGTFHDVK VISEDKANNG TKVVQFEIDE FSKKYNMQMH ILIPAIKYDH KYQVQFEIDA SAIEQKPKFS DVPTWAQESV QYLVDKEAVH GKPDGTFAPA ESIDRSSAAK ILATVLRLEI KKDAKPSFPD AQNHWATPYI AAVEKAGIVK GDEKGNFNPS GLINRASMAS MLVNAYKLER NENIKLPKEF ADLNNHWGAK YANILIQEKI SIGTDNGWAP NKAVSRAEAA QFIAKADKLK KEMK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTAAGAAAAAATATATGAATGC  

PCR Backward Primer

TTATCCACTTCCAATGCTATTTCATTTCTTTCTTCAATTTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 24, 2010