Clone

Target

IDP00464  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

319  

Reference Sequence Used

MDYSKIIKEV GRGKNHARDL DQPTAYALYK AMLAGQVPAL ELGGILIAFR IKGESEQEIL GFYQAMQEQV MSLRAPVDRP LPVVIPSYNG ARKQANLTPL LALLLSRLGL PVVVHGVTED SSRVTSAEIF QQLNIPWSLS VQEAQQRLDN GLPVFIPVSA LSMQLDQQLQ QRWRMGVRNS SHTLAKLATP FTHDKALRVA SVSHPEYMQK VGGFFQAISA PGLLLQGTEG EVYANPLRCP PIHYINDGEQ TILLDRSSES VVPELPLSKD AVITARWIEG CLAGEQPIPD SILKQLACCL VAAGQADSVE LALQQIKIV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MDYSKIIKEV GRGKNHARDL DQPTAYALYK AMLAGQVPAL ELGGILIAFR IKGESEQEIL GFYQAMQEQX MSJRAPXDRP LXXVIPSYXG ARKQANLTPL LALLLSRLGL PVVVHGVTED SXRXTSAEIF QQLNIPWSLS VQEAQQRLDN GLPVFIPVSR TVYAIGSAIT TTLAYGGLET VATPWQSWQH RLLMIKRCAL PASLILNICK KWGGSSKQLV LRGYCYRAPK ARFMLIHCAA HRFIISMMAN RQFYSIVHQN RLSLSYRCRR MP*LRHVGLK AVWPESSLFQ TRF*NS*LVV WSRQGRLILS NWRCNKLRLC  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGATTACAGTAAAATTATCAAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACACAATCTTAATTTGTTGCAA  

Shipped To Lab

ANL  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008