Purification

Target
IDP01178  
Purification ID
A21220  
Expression
M9 minimal (Sep 02, 2014)  
Experiment Date Start
Sep 04, 2014  
Experiment Date End
Sep 06, 2014  
Person
Rory Mulligan  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
0  
Final Volume (μL)
10000  
Shipped To Lab Id
ANL  
Shipped Date
Sep 06, 2014  
Inserted
Feb 21, 2017