Purification

Target
IDP96139  
Purification ID
A22900  
Expression
M9 minimal + semet (Jan 16, 2019)  
Experiment Date Start
Jan 21, 2019  
Experiment Date End
Jan 29, 2019  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
16  
Final Volume (μL)
900  
Shipped To Lab Id
ANL  
Shipped Date
Jan 23, 2019  
Inserted
Feb 01, 2019