Purification

Target
IDP00115  
Purification ID
A5265  
Expression
M9 minimal + semet (Mar 07, 2009)  
Experiment Date Start
Apr 17, 2009  
Experiment Date End
Apr 19, 2009  
Person
Rory Mulligan  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
47  
Final Volume (μL)
1200  
Shipped To Lab Id
ANL  
Shipped Date
Apr 19, 2009  
Inserted
Aug 03, 2011