Purification

Target
IDP00892  
Purification ID
A4550  
Expression
M9 minimal (Nov 17, 2008)  
Experiment Date Start
Nov 19, 2008  
Experiment Date End
Nov 21, 2008  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
failed  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
0  
Final Volume (μL)
0  
Shipped To Lab Id
ANL  
Shipped Date
Nov 21, 2008  
Inserted
Feb 27, 2012