Purification

Target
IDP01178  
Purification ID
A3054  
Expression
M9 minimal (Oct 12, 2010)  
Experiment Date Start
Oct 18, 2010  
Experiment Date End
Oct 20, 2010  
Person
Magdalena Makowska-Grzyska  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
uncleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
1  
Final Volume (μL)
28000  
Shipped To Lab Id
ANL  
Shipped Date
Oct 20, 2010  
Inserted
Feb 27, 2012