Purification

Target
IDP00438  
Purification ID
A8497  
Expression
M9 minimal + semet (Oct 07, 2008)  
Experiment Date Start
Oct 20, 2008  
Experiment Date End
Oct 22, 2008  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
failed  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
double band  
SeMet Status
semet  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
4  
Final Volume (μL)
15000  
Shipped To Lab Id
ANL  
Shipped Date
Oct 22, 2008  
Inserted
Feb 29, 2012