Annotation

Description

SufE is one of the components of the suf operon. The proteins in this operon are implicated in Fe-S cluster assembly under iron limitation and oxidative stress conditions. SufE protein has been shown to bind tightly to SufS in E.coli, the other protein in suf operon, and significantly stimulate its cysteine desulfurase activity. SufS catalyzes the conversion of L-cysteine to L-alanine and sulfide through the intermediate formation of a protein-bound cysteine persulfide in the active site. (Synonyms NifS,CsdB,SufS) (E.C. 2.8.1.7) 

Functional assignment

 

Structure information

Unit cell parameters

Space Group

P 1 21 1  

Unit Cell

a=31.79Å, b=42.62Å, c=45.01Å
α=90.00, β=98.15, γ=90.00 

Solvent content

34.58  

Matthews coefficient

1.88  

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

40.00-1.76Å (1.81-1.76Å)  

R_all(%)

18.8 

R_work(%)

18.5 (25.8) 

R_free(%)

24.0 (27.5) 

Num. observed reflections

11663 (746) 

Num. R_free reflections

559 (38) 

Completeness(%)

97.7 (84.7) 

Model parameters

Num Atoms

1186  

Num Waters

62  

Num Hetatoms

0  

Model mean isotropic B factor

16.174Ų  

RMSD bond length

0.020Å  

RMSD bond angle

1.638°  

Filename uploaded

IDP0875-0128-3.pdb (uploaded on Feb 02, 2009 6:04 PM)  

Inserted

Feb 02, 2009