Annotation

Description

The SARS-CoV-2 open reading frame 8 (ORF8) encodes an accessory protein of unknown function located in the SARS-CoV-2 genome between the reading frames for M and N and just downstream of ORF7a. The product of ORF8 is 121 amino acids in length. The first 15 amino acids encode an n-terminal signal peptide for transport into the endoplasmic reticulum (ER). The ability of ORF8 to associate with ER proteins in immunoprecipitation experiments suggests it may also localize to the ER. However, the protein lacks a discernable transmembrane region or known ER retention signal. The ORF8 encoded protein assembles as a disulfide-linked homodimer. ORF8 deletion events have been observed in SARS-CoV-2, but as yet these represent a minority of the virus population, suggesting the protein might be functionally important for SARS-CoV-2 spread.  

Functional assignment

viral 

Structure information

Unit cell parameters

Space Group

P 43 21 2  

Unit Cell

a=50.65Å, b=50.65Å, c=168.06Å
α=90.00, β=90.00, γ=90.00 

Solvent content

 

Matthews coefficient

 

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

42.02-1.61Å (0.00-0.00Å)  

R_all(%)

20.4 

R_work(%)

20.2 (0.0) 

R_free(%)

23.4 (0.0) 

Num. observed reflections

29522 (0) 

Num. R_free reflections

1154 (0) 

Completeness(%)

99.6 (0.0) 

Model parameters

Num Atoms

1557  

Num Waters

261  

Num Hetatoms

4  

Model mean isotropic B factor

32.210Ų  

RMSD bond length

0.000Å  

RMSD bond angle

0.000°  

Filename uploaded

pdb_extract_coord_27999.cif (uploaded on Dec 09, 2020 4:08 PM)  

Inserted

Dec 09, 2020