Expression

Target
IDP05591  
Expression ID
A3019  
Protein Clone
pMCSG7 (Oct 20, 2010)  
Experiment Date Start
Apr 19, 2011  
Experiment Date End
Apr 21, 2011  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)magic  
Media Type
M9 minimal + semet  
Media Volume
2000 mL  
Growth Temperature
37 °C  
Induction Temperature
16 °C  
Induction Reagent
1 mM IPTG  
Experiment Type
production  
Expression Level
100.0 %  
Solubility Level
70.0 %  
Inserted
Jul 29, 2011  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date