Expression

Target
IDP91749  
Expression ID
J4  
Protein Clone
pMCSG7 (Apr 29, 2013)  
Experiment Date Start
Feb 07, 2013  
Experiment Date End
Feb 07, 2013  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)-Magic  
Media Type
2xYT  
Media Volume
0.15 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
1mM IPTG  
Experiment Type
assay  
Expression Level
30.0 %  
Inserted
Mar 28, 2013