Expression

Target
IDP00148  
Expression ID
J7  
Protein Clone
pMCSG53 (Mar 04, 2013)  
Experiment Date Start
Mar 16, 2013  
Experiment Date End
Apr 04, 2013  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)-Magic  
Media Type
2xYT  
Media Volume
0.15 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
1mM IPTG  
Experiment Type
assay  
Expression Level
30.0 %  
Solubility Level
100.0 %  
Inserted
Jun 27, 2013