Expression

Target
IDP91025  
Expression ID
A11091  
Protein Clone
pMCSG68 (Oct 10, 2017)  
Experiment Date Start
Dec 12, 2017  
Experiment Date End
Dec 14, 2017  
Person
Grazyna Joachimiak  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21-Gold(DE3)  
Media Type
M9 minimal + semet  
Media Volume
1000 mL  
Growth Temperature
37 °C  
Induction Temperature
18 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
80.0 %  
Solubility Level
90.0 %  
Inserted
Dec 20, 2017