Expression

Target

IDP00044  

Expression ID

U168  

Protein Clone

pMCSG7 (Apr 21, 2008)  

Experiment Date Start

Feb 13, 2009  

Experiment Date End

Feb 14, 2009  

Person

Majka Klimecka  

Lab

University of Virginia  

Protocol

uva_expression_semet_1  

Status

success  

Expression Organism

E.coli  

Expression Strain

E.coli BL21 DE3 Codon Plus RIPL  

Media Type

SeMet  

Media Volume

4000 mL  

Growth Temperature

37 °C  

Induction Temperature

20 °C  

Induction Reagent

IPTG  

Experiment Type

production  

Expression Level

80.0 %  

Solubility Level

80.0 %  

Inserted

Feb 18, 2009