Expression

Target

IDP98083  

Expression ID

N2214  

Protein Clone

pMCSG53 (Aug 21, 2020)  

Experiment Date Start

Apr 19, 2021  

Experiment Date End

Apr 22, 2021  

Person

Grant Wiersum  

Lab

Northwestern University  

Protocol

NU_SelMet_expression  

Status

success  

Expression Organism

E. Coli  

Expression Strain

Bl-21 Magic  

Media Type

Selenomethionine  

Media Volume

3000 mL  

Growth Temperature

37 °C  

Induction Temperature

25 °C  

Induction Reagent

IPTG  

Experiment Type

production  

Expression Level

100.0 %  

Solubility Level

100.0 %  

Inserted

Apr 23, 2021