Expression

Target
IDP97826  
Expression ID
N2222  
Protein Clone
pMCSG53 (Jan 13, 2021)  
Experiment Date Start
May 10, 2021  
Experiment Date End
May 14, 2021  
Person
Grant Wiersum  
Lab
Northwestern University  
Protocol
Autoinduction HTMC1-HTMC2  
Status
success  
Expression Organism
E. Coli  
Expression Strain
Bl-21 Magic  
Media Type
Selenomethionine  
Media Volume
3000 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
IPTG  
Experiment Type
production  
Expression Level
100.0 %  
Solubility Level
100.0 %  
Inserted
May 26, 2021  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date