Clone

Target
IDP00160  
Clone Suffix
1
Experiment Start Date
Jun 06, 2008  
Experiment End Date
Jun 07, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
152  
Reference Sequence Used
MNIKLSFISI AFLSLSFNVA ANEFEKSQEH YKSVTDLKNK IEILELEKKI TELSGEIRNA RMPKIDKSAP VLSPQPVVKS SEELQKSIEH IEEELKVELA YLVNNGQQKK YTFNLNGKLI TLVNGDFVNG WKFIEDQNKI QFSKGNKVID VN  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MNINYHLFQL LFYR*VLMLL LTNLKNLKST TKV*LI*KTK SKY*S*KRK* QS*VARFAMQ ECQK*ISLHP FYLLSL**NR QKNYKKVSNI *KKSSKWNWL IWLTMVNKKN IHSILMANLS HWLMVIL*MV GNSLKTKIKF NFPRAIR*SM SI  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAATATCAAACTATCATTTATTTC  
PCR Backward Primer
TTATCCACTTCCAATGTCAATTGACATCGATTACCTTAT  
Shipped To Lab
ANL  
Shipped Date
Jun 30, 2008  
Inserted
Aug 08, 2008  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-06-19 2008-06-20 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli M9 minimal 1mM IPTG assay
2008-07-16 2008-07-18 Rory Mulligan Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 1 mM IPTG production
2008-10-20 2008-10-22 Rory Mulligan Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production