Clone

Target

IDP00644  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

381  

Reference Sequence Used

MLTEQEKDII KQTVPLLKEK GTEITSIFYP KMFKAHPELL NMFNQTNQKR GMQSSALAQA VMAAAVNIDN LSVIKPVIMP VAYKHCALQV YAEHYPIVGK NLLKAIQDVT GLEENDPVIQ AWAKAYGVIA DVFIQIEKEI YDQMMWIGFK PFKITNIKQE SEDIKSFTVE TEEYDFSEFT PGQYITVDVS SDKLPYRAKR HYSIVSGEKN HLTFGVKRDV TTEHEGEVST ILHDEIKEGD MINLAAPVGG FVLENTTEPQ LFLGSGIGVT PLVAMYEAAS AKGLDTQMVQ VAENEQHLPF KDNFNSIASH HDNAKLYTHL KDKQGYIGAE ELQVFLANKP EIYICGGTKF LQSMIEALKS LNYDMDRVHY ETFIPRLSVA V  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTTACAGAACAAGAGAAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTATACTGCAACGCTTAGTCTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008