Clone

Target

IDP00050  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

742  

Reference Sequence Used

MKRLLVIGIM YTMFFLIGNI HLHADERTNV KEITSLEEPT WIFQAGISKG KYHDRQDLGF ILQRNTPLKV RQTNPNFKDK LTVRLLSNDS KNEKSIQVGN EWITIQGDTP LVPFIDTPYG EEPALLEYQV GNESATKPLP IYKQQGSVSQ FFSTWDQFDG EYALIQGESF QLFIPKKDKE LVRSLKDFQS LDELIAYYED IFAMYDSIIG LDGSTVENKK SQNRYFLKAD ISGAGGAYYG ANWTANSTDS TKMWLDKLSW GTLHEIAHGY QAGFDNQGIF TGEVSNNLFG VQYQYSKYGK KADQVGWLFN FGKKEQVERN LYNALMKENK NYDDLDLRQK LILLTMAKQK AGDEAFAKMY QGYRKLASNA AFKKGDHSLP DLMNQYYSEN VQVDFTPVFE RWGFKLNHKQ IEMNRAKGYP AVTSLAYIVP ESQLAKARAL VDPDIPINSN FEIVRNQQIA SLGLKGNLHI HLNTNEIDTL KGGKIKLKEG NTVIQEKTIE TADINLQDVP NGIYTVEISG GKTDSMYHFS TYYAYVKEKD NSLTIDVNEM KVSNLVNETI QFLGLGDDQF AELNTDLEQE RAVFTVTTKT PHSYYAGEKY ASIELFNEKG EKIYTKEMEG TNVTVVKDII PLKEGYRIKI YHDEVKKRLT SKATIINPMN KTNEIIMTKW GLKNTYLKNN PEENLMKRID EEMEVIISNP LLKEIPMQKL EMKKNVWMAI NMLSEPQKIT YINKYKDSLY NE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAAGGCTACTTGTTATAGG  

PCR Backward Primer

TTATCCACTTCCAATGTCACTCATTATATAAACTATCTTTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008