Clone

Target

IDP00193  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

765  

Reference Sequence Used

MIISPLFLKD KSNSEDDASW VSRMMPVDPE RGFPLNSHRS WHGGIHIPHT DSGGTPEKIR AIADGTVHSL RQPVGDKKGI PPYNYNGATD CGYVLLKHET EIGSGENAKV VYFSLYMHLA SIDAAVKTGE KIYRKDPIGT VGQVDGGNAV HFQIFCDDTN LTKLVGRTTP ELDLTKNGRT DAVYGDMYFY LPTGTKFYSH APANNATTTT EAETYTSTEP LFVSMSFDKG KCTMLTRKKG VFSIGKYSEV GEKLENTDGA DYEYNVYAQA KKLYPQSQSA GYELLRFGRV INTDYETLVP ADAPLWRTIS YPGGKGVVNL ALPAVKKFSD GDFPHWTGWQ LIDDDTDENS QCNSEQVLCR IGDDMQGLIC HLPLEWDAST LEKRLSWLKT TVINESLANQ KASHQVDDTG NNASGYAFDN INPDMSVLNA QSTPSFEQAA KDTAMAEADW NALIEHAKAL CFDVSGLPVG RVWHFNPISF IAHFRKCGWL DPEKIKSILT SALNKKRNVR KITLIEKASD SNYTFLNLAM RKYNILTPNR QSHFLGQGAV ESDSLTAMQE VSQEQVIVNG KQTGGGIVIK STLNESELGH WYGSEPSELD IYYSGNKYNK NGGLIAGSYS WSNGNCGDVD AQKFRGRGFK MLTGRDKYAD YWVYRGWLLK SSFDAYWWDD VKYKSKNLKA MKKRPAIIDD PHKITASPYN CIDSGSFFIT CFRRSTVKEI DKDQAESADD SVVTKVTQAI NGGDIGLSQR KVMTNNAKGK LNDKY  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTATCAGCCCTTTATTTTTA  

PCR Backward Primer

TTATCCACTTCCAATGTTAATATTTATCATTTAATTTTCCCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008