Clone

Target

IDP00025  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

309  

Reference Sequence Used

MSPINTIIKT TNLTKVYGKQ KSVDNLNINV QQGEIYGFIG RNGAGKTTTI RMLLGLIKPT SGTIEIFGED LFQNQKDILS RIGSIVEVPG FYENLTAKEN LLINAKIIGV YKKNAIEDTL EIVGLQHETK KLVGKYSLGM KQRLGIARAL LHYPELLILD EPTNGLDPIG IKEMRKLIHS LAQERNITIL ISSHILAEVE QLVDRIGIIH EGRLLEEVSL DKLRKANRKY IEFQVNNENK AAMLLENQFQ IFDYEVHDEG NIRIYSHFGQ QGYINRTFVL NDIEVLKMMI SEDRLEDYFT KLVGGGTIG  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTCTCCTATAAACACAATTATA  

PCR Backward Primer

TTATCCACTTCCAATGTTAACCAATTGTTCCACCTCC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008