Clone

Target

IDP00073  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

182  

Reference Sequence Used

MSKQLAAQVP AEPVVLGKMG SSYGIRGWLR VFSSTEDAES IFDYQPWFIQ KAGQWQQVQL ESWKHHNQDL IIKLKGVDDR DAANLLTNCE IVVDSSQLPA LEEGDYYWKD LMGCQVVTAE GYDLGKVIDM METGSNDVLV IKANLKDAFG IKERLVPFLD GQVIKKVDLA TRTIEVDWDP GF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGAGCAAGCAACTCGCCG  

PCR Backward Primer

GGTTCTCCCCACGAAAACCAGGATCCCAGTCTAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011