Clone

Target

IDP00154  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

545  

Reference Sequence Used

MTTNYIFVTG GVVSSLGKGI AAASLAAILE ARGLKVTMMK LDPYINVDPG TMSPTQHGEV FVTEDGAETD LDLGHYERFI RTKMTKRNNF TAGRVYADVL RKERRGDYLG ATIQVIPHIT NAIKDRVIAG SEGHDIAIVE VGGTVGDIES LPFMEAIRQL AIEVGREHAM FMHLTLVPYL AAAGEVKTKP TQHSVKELLS IGIQPDILVC RSDRMIPANE RKKIALFCNV PEKAVISMKD VDSIYKIPQL IRSQGLDDLV CARFGINAPE ADLSEWEQVI YEEANPTGEV TIGMVGKYTE LPDAYKSVNE ALKHAGLKNR LSVTIKYVDS QDVETKGTDV LNGLDAILVP GGFGDRGIEG KIRAAQYARE NKIPYLGICL GMQVALIEYA RNVAGMEGAH STEFNKNTKY PVVGLITEWV DGEGNVEERS EKSDLGGTMR LGSQLCHLEK GTKAYELYGS ATIHERHRHR YEVNNLLRPQ IEKAGLKVSG LSADKKLVEV IENPAHPWFV AAQFHPEFTS TPRDGHPLFA GFVKAAGQFQ RGELK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACGACAAATTATATTTTTGTTA  

PCR Backward Primer

TTATCCACTTCCAATGTTACTTCAATTCACCGCGTTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008