Clone

Target

IDP04378  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

482  

Reference Sequence Used

MLKFKKLIAL TACAMLTTSV ALTGCGADKT ANAGEGETVK LTWYTIGQTP KDLDMVQEKA NEYLKEKINA TIDMKFIDYG DYTQKMGVII NSGEPYDLAF TCSWANPYLE NARKGAFLEI DELLETKGKE MKSVIDERFW EGAKIDGKTY AVPNQKEIGV APMWVFTKEY VDKYNIPYQD IHTLEDLEPW LKVIHENEPD VTPLYITKGF SAPAYFDQLV DPVGVEYGDE SLKIKNMFET DKMKSELETL QKYYDAGYIN ADSATAKDDK AVKRFVTKAD GQPYADGLWS KDLGYEVVSS PIMDTHITNG STTGSMIAIS KTSEHPEKAM EFLNLLNTDV YLRNLLNYGI EGTHYEKTSD TQIKLTDKAK DYSVGYYTLG NLFITYTLDN EPVDKWKEFE AFNDASVESP ALGFKFNTEK VSNQIAAINN VLEEFKATIY SGSVNEAEYL DKMNKKLKEV GIDEVISEMQ SQIDAWKAEN GK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTAAAATTCAAAAAACTAATAGC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTTCCATTTTCAGCTTTCC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011