Clone

Target

IDP04383  

Clone Suffix

2 The clone is not available in BEIR.

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

806  

Reference Sequence Used

MFVSYRWLQE YVDIKDVTAQ ELADKITKSG IEVEGVEVLN KGVKGVVVGH VLECEKHPEA DKLSKCLIDI GEEEPVQIIC GAANIAKGLK VPVAKVGAVL PGNFKIKKAK LRGEASHGMV CALQELGIDG KLVSKEYADG IFIFPSDAEV GADALEILNL HDEVLELGLT PNRADCLNML GVAYEVAAIY GREVKLPAID LQETAEKTSD YISVSVEAKE ENPLYIAKMV KNVKIGPSPM WMQTRLMAAG IRPISNVVDI TNYILMEYGQ PLHAFDYDKL GSKEIVVRLA KEGEKIETLD DQERTLQSHH LVITNGTKAL AVAGVMGGAD SEVTNETVNV LIESAYFAGQ TVRRTSKDLG LRSESSARFE KGIDPTRTFE AIQHAAALMA KYAGGEALEG VVEADNLQVQ ERTVSVTAEK VNRVLGTNIS ASEMGTMFTN LKFPFTEVEG TFHVNVPARR PDITISEDLV EEVGRLYGYD HIPVTLPSGT MTRGKLTAAQ TKRRKVRRFL EGAGLYEAIT YSLTSADKAK QYMVEPNEKA PVNLALPMSE ERSQLRLSLV PQLLEAVSYN VARKNDSVAL YEVGSIFLPT EAGELPKEEQ HLAGVMTGLA LHHAWQGEKK VVDFFVVKGV LEGLFDVLGV SNQITYAPAK REGMHPGRTA DIVLDGEVIG FIGQLHPEAE KQLDVKNTFV FELSLVKVFG TDAEETYYSA IPRFPSMTRD MAVVVTKETK AGEMKQVIAE AGGELLKDVT LFDLYEGEKM EEGKKSLAFS MNYFDPERTL TDEEVTEAHN RVLTAVEEKF GAELRK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MFVSYRWLQE YVDIKDVTAQ ELADKITKSG IEVEGVEVLN KGVKGVVVGH VLECEKHPEA DKLSKCLIDI GEEEPVQIIC GAANIAKGLK VPVAKVGAVL PGNFKIKKAK LRGEASHGMV CALQELGIDG KLVSKEYADG IFIFPSDAEV GADALEILNL HDEVLELGLT PNRADCLNML GVAYEVAAIY GREVKLPAID LQETAEKTSD YISVSVEAKE ENPLYIAKMV KNVKIGPSPM WMQTRLMAAG IRPISNVVDI TNYILMEYGQ PLHAFDYDKL GSKEIVVRLA KEGEKIETLD DQERTLQSHH LVITNGTKAL AVAGVMGGAD SEVTNETVNV LIESAYFAGQ TVRRTSKDLG LRSESSARFE KGIDPTRTFE AIQHAAALMA KYAGGEALEG VVEADNLQVQ ERTVSVTAEK VNRVLGTNIS ASEMGTMFTN LKFPFTEVEG TFHVNVPARR PDITISEDLV EEVGRLYGYD HIPVTLPSGT MTRGKLTAAQ TKRRKVRRFL EGAGLYEAIT YSLTSADKAK QYMVEPNEKA PVNLALPMSE ERSQLRLSLV PQLLEAVSYN VARKNDSVAL YEVGSIFLPT EAGELPKEEQ HLAGVMTGLA LHHAWQGEKK VVDFFVVKGV LEGLFDVLGV SNQITYAPAK REGMHPGRTA DIVLDGEVIG FIGQLHPEAE KQLDVKNTFV FELSLVKVFG TDAEETYYSA IPRFPSMTRD MAVVVTKETK AGEMKQVIAE AGGELLKDVT LFDLYEGEKM EEGKKSLAFS MNYFDPERTL TDEEVTEAHN RVLTAVEEKF GAELRK  

PCR Forward Primer

GTCTCTCCCATGTTCGTATCATATCGTTGGT  

PCR Backward Primer

GGTTCTCCCCACGCTTACGTAACTCCGCACCAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011