Clone

Target

IDP04398  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

1305  

Reference Sequence Used

MSQEYTEDKE VTLKKLSNGR RLLEAVLIVV TILAAYLMVA LVSFNPSDPS WSQTAWHEPI HNLGGSIGAW MADTLFSTFG VLAYAIPPIM VIFCWTAFRQ RDASEYLDYF ALSLRLIGTL ALILTSCGLA ALNIDDLYYF ASGGVIGSLF SNAMLPWFNG VGATLTLLCI WVVGLTLFTG WSWLVIAEKI GAAVLGSLTF ITNRSRREER YDDEDSYHDD DHADGRDITG QEKGVVSNKG VVSNNAVVGA GVAASSALAH GDDDVLFSAP SVTDSIVEHG SVVATGTETT DTKATDTNDE YDPLLSPLRA TDYSVQDATS SPIADVAVEP VLNHDAAAIY GTTPVMTNTA TPPLYSFELP EESLPIQTHA APTERPEPKL GAWDMSPTPV SHSPFDFSAI QRPVGQLESR QPGSNQSGSH QIHSAQSSHI SVGNTPYMNP GLDAQIDGLS TTSLTNKPVL ASGTVAAATA AAAFMPAFTA TSDSSSQIKQ GIGPELPRPN PVRIPTRREL ASFGIKLPSQ RMAEQELRER DGDETQNPQM AASSYGTEIT SDEDAALQQA ILRKAFADQQ SERYALSTLA EQSSITERSP AAEMPTTPSQ VSDLEDEQAL QEAELRQAFA AQQQHRYGAT GDTDNAVDNI RSVDTSTAFT FSPIADLVDD SPREPLFTLS PYVDETDVDE PVQLEGKEES LLQDYPEQVP TYQPPVQQAH LGQSAPTQPS HTQSTYGQST YGQSTYGQST PAPVSQPVVT SASAISTSVT PTSIASLNTA PVSAAPVAPS PQPPAFSQPT AAMDSLIHPF LMRNDQPLQK PTTPLPTLDL LSSPPAEEEP VDMFALEQTA RLVEARLGDY RVKAEVVGIS PGPVITRFEL DLAPGVKASR ISNLSRDLAR SLSAIAVRVV EVIPGKPYVG LELPNKHRQT VYLREVLDCA KFRENPSPLA IVLGKDIAGQ PVVADLAKMP HLLVAGTTGS GKSVGVNAMI LSILYKATPD DVRFIMIDPK MLELSVYEGI PHLLTGVVTD MKDAANALRW CVGEMERRYK LMSALGVRNL AGYNERVAQA EAMGRPIPDP FWKPSDSMDI SPPMLVKLPY IVVMVDEFAD LMMTVGKKVE ELIARLAQKA RAAGIHLVLA TQRPSVDVIT GLIKANIPTR IAFTVSSKID SRTILDQGGA ESLLGMGDML YMAPNSSIPV RVHGAFVRDQ EVHAVVNDWK ARGRPQYIDS ILSGGEEGEG GGLGLDSDEE LDPLFDQAVN FVLEKRRASI SGVQRQFRIG YNRAARIIEQ MEAQQIVSTP GHNGNREVLA PPPHE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGCCAGGAATATACAGAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCATGGGGTGGTGGTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011