Clone

Target

IDP04418  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

386  

Reference Sequence Used

MNISNIKIML LGSGELGKEF IIAAQRLGIH TIVVDRYKNA PAMQVAHESY VIDMLNSDAL EQLILAKNPT YIVPEIEAIN TDSLVKLEAH NFNIIPCAKA AKLTMDRQGI RALAAQQLNL QTSKFAFANS EQEYLDVIQS IGLPFVIKPV MSSSGKGQSI VKEHNEIKKA WDYAQNGSRG HAKGVIVEQF IDFDYEITLL TVRHKDGTSF CDPIGHIQKD GDYRFSWQPH TMPDTALAKS QEIAKEITDA LGGYGVFGVE LFIKGDEVFF NEVSPRPHDT GMVTLISQNI NEFELHLRAI VGLPIPDIQT LQPSASAAIL LEGDTANASI CGIDKALADA NVDIRIFGKK EIHGKRRMGV VLAKAQNTHI ALETSKQALA HIHLTK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGAATATTTCTAATATCAAAATTATG  

PCR Backward Primer

GGTTCTCCCCACGTTTAGTAAGATGAATATGTGCTAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011