Clone

Target

IDP04509  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

350  

Reference Sequence Used

MLKRKKLLAL TLTALVSMGV LAGCGQKNQE AKAEETGSGE KVVNVYSSRH YDIDKQLFKD FESDTGIKVN LVDGKSDELI ERMAREGDNT SADVFLTVGA ENIAQLNEKD LIKDISSEKI NENIPSEYRG KNWVGLTSRA RIIVYNKEKV NPETIKTYND LTKDEFKGKV LTRSSSSSYN NALLSSFIQL DGAEKAKEWA QGIVNNFART PEGNDRDQAK AIMAGIGDVG IMNSYYIAKM ISSSDAEEAK VGKEIGVLFP ENTHLNLSFG AVTKNAKNEE NAVALLEYLT EEKAQKAYVD ENGEFALNTK VGKSELQKSW GEFTVQNLNY EILGESKQEA TLIFDQVGWK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTAAAAAGAAAAAAATTATTAGC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTCCATCCTACTTGATCAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011