Clone

Target

IDP90547  

Clone Suffix

2 The clone is not available in BEIR.

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

243  

Reference Sequence Used

MTQIIPALDL IDGEVVRLVK GDYEQKKVYK YNPLKKFKEY EKAGAKELHL VDLTGAKDPS KRQFALIEKL AKEVSVNLQV GGGIRSKEEV KALLDCGVKR VVIGSMAIKD ATLCLEILKE FGSEAIVLAL DTILKEDYVV AVNAWQEASD KKLMEVLDFY SNKGLKHILC TDISKDGTMQ GVNVRLYKLI HEIFPNICIQ ASGGVASLKD LENLKGICSG VIVGKALLDG VFSVEEGIRC LAN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTQIIPALDL IDGEVVRLVK GDYEQKKVYK YNPLKKFKEY EKAGAKELHL VDLTGAKDPS KRQFALIEKL AKEVSVNLQV GGGIRSKEEV KALLDCGVKR VVIGSMAIKD ATLCLEILKE FGSEAIVLAL DTILKEDYVV AVNAWQEASD KKLMEVLDFY SNKGLKHILC TDISKDGTMQ GVNVRLYKLI HEIFPNICIQ ASGGVASLKD LENLKGICSG VIVGKALLDG VFSVEEGIRC LAN  

PCR Forward Primer

GTCTCTCCCATGACTCAAATCATACCCGCA  

PCR Backward Primer

GGTTCTCCCCACGATTTGCTAGACATCTTATCCCT  

Shipped To Lab

ANL  

Shipped Date

Sep 19, 2011  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011