Clone

Target

IDP91015  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

673  

Reference Sequence Used

MKMTRLYPLA LGGLLLPAIA NAQTSQQDES TLVVTASKQS SRSASANNVS STVVSAPELS DAGVTASDKL PRVLPGLNIE NSGNMLFSTI SLRGVSSAQD FYNPAVTLYV DGVPQLSTNT IQALTDVQSV ELLRGPQGTL YGKSAQGGII NIVTQQPDST PRGYIEGGVS SRDSYRSKFN LSGPIQDGLL YGSVTLLRQV DDGDMINPAT GSDDLGGTRA SIGNVKLRLA PDDQPWEMGF AASRECTRAT QDAYVGWNDI KGRKLSISDG SPDPYMRRCT DSQTLSGKYT TDDWVFNLIS AWQQQHYSRT FPSGSLIVNM PQRWNQDVQE LRAATLGDAR TVDMVFGLYR QNTREKLNSA YDMPTMPYLS STGYTTAETL AAYSDLTWHL TDRFDIGGGV RFSHDKSSTQ YHGSMLGNPF GDQGKSNDDQ VLGQLSAGYM LTDDWRVYTR VAQGYKPSGY NIVPTAGLDA KPFVAEKSIN YELGTRYETA DVTLQAATFY THTKDMQLYS GPVRMQTLSN AGKADATGVE LEAKWRFAPG WSWDINGNVI RSEFTNDSEL YHGNRVPFVP RYGAGSSVNG VIDTRYGALM PRLAVNLVGP HYFDGDNQLR QGTYATLDSS LGWQATERMN ISVYVDNLFD RRYRTYGYMN GSSAVAQVNM GRTVGINTRI DFF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAATGACACGGCTTTATC  

PCR Backward Primer

TTATCCACTTCCAATGTCAGAAGAAATCAATTCGCGTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011