Clone

Target

IDP91214  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

540  

Reference Sequence Used

MTARPRAVII GMMGAGKTRV GKEVAHMLRL PFADADVEIE REVGMKIPSY FEEYGEPAFR EVEADLIADM LEDFDGIFSL GGGAPMTSST QHALASYIDH GGRVVYLDAD PAEAMERANR GGGRPMLNGN ANSRWKKLFK QRDPVFREVA NVHVHTRGLT PQGAAKKVID MVSERAVHVT GAAIEPYDVV IGEGAMNHLA DVLGPKPAKI ALIHTQSVQR HSDRARALLR QGGYEVSDIV IPDAEPGKTI TVANGIWERL GNEGFTRSDA VVGLGGGAAT DLAGFVAATW MRGVRYVNCP TSLLAMVDAS TGGKTGINTP QGKNLVGSFY TPAGVLADTK TLATLPNDIF IEGLGEVAKS GFIRDPEILH ILEDHAAELR AFVGSTFLGS PLEDVVAELI ERTVKVKVYH VSSDLKEKGL REFLNYGHTM GHAIEKLEHF RWRHGNAVAV GMVYAAELAH LIGYIDQDLV DYHRSLLASL GLPTSWHGGS FDDVLALMHR DKKARGNELR FVVLDEIGHV VHLDNPPADA VEEAFHRIQQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACCGCACGCCCCCGC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGCTGGATACGATGGAAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011