Clone

Target

IDP00178  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

256  

Reference Sequence Used

MTRLYWIALQ SIWIKEITRF ARIWIQTLVP PVITMSLYFV IFGNLIGARI GDMGGFDYMQ FIVPGLIMMA VITNAYSNVA ASFYGAKFQR SIEELLVAPV PTHIVIIGYV GGGVARGICV GILVTIISLF FVPLHVHSWS MIALTLILTA ILFSLGGLLN AVFAKTFDDI SLVPTFVLTP LTYLGGVFYS LSLLPPFWQA VSKLNPIVYM ISGFRYGFLG ITDVSLAYTI GVLVVFIAVF YAWAWYLIER GTGLRT  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACCCGCCTGTATTGGAT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGGTACGTAACCCGGTTC  

Shipped To Lab

NU  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008