Clone

Target

IDP91343  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 21, 2011  

Experiment End Date

Jul 21, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

178  

Sequence End

518  

Reference Sequence Used

WSLLLLSVAI SYSSTRRLES LSAEARKVFD NPLMELVYNK KVDDISEIKL ALKMRQSELN AVVGRIQDSN QQLIDSARNS SSNSDKASHN LKGQTHETEQ VAAAITEMHS TANEIAQNAQ SASDATDQAH SAASEGMITV QDTVKAIKLL AGQLDETSDV VSQLAQHGKT IGDVLIIIQG VAEQTNLLAL NAAIEAARAG EQGRGFAVVA DEVRKLAQRS HESTEEIQKV IGLIQSSTQK AVSSMSEGTH LAEQCVSSAD SSGQKLQILL DQVTDISDRN NQIATAVEEM ARVTEDMSSS VQSISDACSA TNVLSSDTCH QCNALVKSLD SQGKLVSQFR R  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGTGGTCACTTCTCTTACTGTCT  

PCR Backward Primer

TTATCCACTTCCAATGTCAGCGTCTAAATTGAGAAACC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011