Clone

Target

IDP91430  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

408  

Reference Sequence Used

MIRTFADLDL DPNLLAAIEE MGYERPTQIQ AEAIPQALEG RDILASAPTG TGKTAAFVLP ALQYLQDFPR RKPGPARVLI LTPTRELAMQ VADQARALAK NTSLNIFTIT GGVQYQEHAD ILATTQDIVV ATPGRLMEYI QAERFDCRAI EWLILDEADR MLDMGFAPTV DRLSNECRWR KQTMLFSATL EGKGVEGFTA DLLNDPADID AEPSRRERKK ITQWYYRADN MEHKLALLKD IINNQAERTI VFLKTRERLA DLRAHLESEQ VNCAWIQGEM PQDRRNNAIA RFRDGSVNVL LATDVAARGI DLPDVSHVVN FDMPRSADVY LHRIGRTARA GKKGNAISIV EAHDQPMMDR VARYVKEDIK ERFVKELRPQ HKKPVFKKKK KKDDKKTSVK KKSTKKKK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATCAGAACCTTTGCCGAT  

PCR Backward Primer

TTATCCACTTCCAATGTTACTTTTTCTTTTTGGTGCTTTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011