Clone

Target

IDP91559  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

223  

Reference Sequence Used

MDNLIGTPPN HAVPHNYIDM EQMEYLCHLN RFSKLSNDFL INGPREDLFT YKYVLDGSFS NLQHLLPKGQ LQKIQQRLSS LMHKNMFHCF HVFLLKLCSI ESIPLPNADY AFFDDEMPFT LTDEQIENIS FLNAYHKEKK GNNDIVTFDF MSADHHYNFS TTIALTNDSF HISSINNHNS QVIFDENIHL HPYELPESSQ WCYQLIKNMI SLHCRYNNNF KIN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGATAATCTTATTGGCACAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTTATCTTGAAATTGTTATTAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011