Clone

Target

IDP91580  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

263  

Reference Sequence Used

MRQLVSIKHK LYAPLKKIDL TAVPLMTVGA VLVFLLGKNK VISPFADDYI FTTDGNIKNE FAVYFALVGA NAALSIYTSA RTIFNDWQLR KAHYANPENR LQEIGAQISE GTQLLTGPVE LEENSNECKW EARPALGAFF EKHPLLWHGV STLLRTTGVI LISTATMSPP FLERVVDLIK DNLSFSMEEQ TIFYGSYAAF EVLTLLLNWV NYHEMQMPLK LFLKEGENNA RLRECKSALF SLLHNDQPVP EPNVEASRGC SIM  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGACAACTCGTTTCAATTAA  

PCR Backward Primer

TTATCCACTTCCAATGTTACATAATAGAACACCCACGA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011