Clone

Target
IDP91767  
Clone Suffix
1 The clone is not available in BEIR.
Experiment Start Date
Jul 22, 2011  
Experiment End Date
Aug 01, 2011  
Person
Olga Kiryukhina  
Lab
Northwestern University  
Protocol ID
Autoinduction HTMC1-HTMC2  
Status
success  
Vector
MCSG7  
Sequence Start
1  
Sequence End
186  
Reference Sequence Used
MPSAHSVKLR PLEREDLRYV HQLDNNASVM RYWFEEPYEA FVELSDLYDK HIHDQSERRF VVECDGEKAG LVELVEINHV HRRAEFQIII SPEYQGKGLA TRAAKLAMDY GFTVLNLYKL YLIVDKENEK AIHIYRKLGF SVEGELMHEF FINGQYRNAI RMCIFQHQYL AEHKTPGQTL LKPTAQ  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MPSAHSVKLR PLEREDLRYV HQLDNNASVM RYWFEEPYEA FVELSDLYDK HIHDQSERRF VVECDGEKAG LVELVEINHV HRRAEFQIII SPEYQGKGLA TRAAKLAMDY GFTVLNLYKL YLIVDKENEK AIHIYRKLGF SVEGELMHEF FINGQYRNAI RMCIFQHQYL AEHKTPGQTL LKPTAQ  
PCR Forward Primer
TACTTCCAATCCAATGCGATGCCAAGCGCCCACAGTG  
PCR Backward Primer
TTATCCACTTCCAATGCTATTGTGCGGTCGGCTTC  
Shipped To Lab
NU  
Shipped Date
Oct 11, 2011  
Secondary Shipped To Lab
NU  
Secondary Shipped Date
Oct 11, 2011  
Inserted
Oct 04, 2011  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-08-02 2011-08-02 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-10-24 2011-10-26 Olga Kiryukhina Northwestern University NU_native_expression success E.coli Terrific Broth IPTG production
2016-09-26 2016-09-28 Olga Kiryukhina Northwestern University NU_native_expression success E. coli Terrific Broth IPTG production