Clone

Target
IDP04364  
Clone Suffix
104
Sequence Comments
IDP04364.1-489.F..pMCSG50  
Experiment Start Date
Jun 01, 2011  
Experiment End Date
Jun 01, 2011  
Person
Magdalena Makowska-Grzyska  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG50  
Sequence Start
1  
Sequence End
489  
Reference Sequence Used
MHMLRIAKEA LTFDDVLLVP AHSTVLPNTA DLRTRLTKNI ALNIPMVSAS MDTVTEARLA IALAQEGGIG FIHKNMSIEQ QAAQVHQVKI FEAGVVTHPV TVRPEQTIAD VMELTHYHGF AGFPVVTENN ELVGIITGRD VRFVTDLTKS VAAVMTPKER LATVKEGATG AEVQEKMHKA RVEKILVVND EFQLKGMITA KDFHKAESKP NACKDEQGRL RVGAAVGAAP GNEERVKALV EAGVDVLLID SSHGHSEGVL QRIRETRAAY PHLEIIGGNV ATAEGARALI EAGVSAVKVG IGPGSICTTR IVTGVGVPQI TAIADAAGVA NEYGIPVIAD GGIRFSGDIS KAIAAGASCV MVGSMFAGTE EAPGEVILYQ GRSYKAYRGM GSLGAMSKGS SDRYFQTDNA ADKLVPEGIE GRIAYKGHLK EIIHQQMGGL RSCMGLTGSA TVEDLRTKAQ FVRISGAGMK ESHVHDVQIT KEAPNYRLG  
PCR Forward Primer
TACTTCCAATCCAATGCCTTGCACATGCTACGAATCGCAAAAGAAG  
PCR Backward Primer
TTATCCACTTCCAATGTTAACCCAGACGGTAGTTTGGTGCTT  
Shipped To Lab
ANL  
Shipped Date
Jun 01, 2011  
Inserted
Nov 04, 2011  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-06-02 2011-06-02 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 1 mM IPTG assay
2011-08-10 2011-08-12 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 0.5 mM IPTG production
2011-08-30 2011-09-01 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 0.5 mM IPTG production