Clone

Target

IDP00069  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Nov 02, 2011  

Experiment End Date

Nov 02, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

408  

Reference Sequence Used

MRRTFKIMLI AGVIAAIGGV IYMAGEALWD KDNAVGPPAS APPPPSVPVA KALSRTLAPT AEFTGFLAAP ETVELRSRVG GTLDAISVPE GRLVSRGQLL FQIDPRPFEV ALDTAVAQLR QAEVLARQAQ ADFDRIQRLV ASGAVSRKNA DDVTATRNAR QAQMQSAKAA VAAARLELSW TRITAPIAGR VDRILVTRGN LVSGGVAGNA TLLTTIVSHN PMYVYFDIDE ATWLKALRHT RSDKNPPVVN MGLTTDNGLP YQGVLDFMGN QMNRSTGTIR ARAVIPDPDG MLSPGLFARI SLPIGEPRET VLIDDLAVSA DQGKNYVLIV GKENQVEYRP VELGQMVDGF RVVTQGVLPG EKIILKGLVR PGMTVAPRLV PMRQNVTDKQ TATLTKADGD SAPKAVRQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGCGTAGAACATTCAAAATTATG  

PCR Backward Primer

GGTTCTCCCCACGTTGGCGCACCGCCTTCGG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 12, 2012