Clone

Target

IDP00226  

Clone Suffix

7

Sequence Comments

 

Experiment Start Date

Nov 16, 2011  

Experiment End Date

Nov 17, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

8  

Sequence End

815  

Reference Sequence Used

TSPVVSVDAL KHSIAYKLMF IIGKDPTIAT QHDWLNATLF AVRDRMVERW LRSNRAQLSQ DVRQVYYLSM EFLLGRTLSN ALLSMGIYDE IEQALDEMGL SLSELLKEEN DPGLGNGGLG RLAACFLDSL ATLALPGRGY GIRYEYGMFS QKIVNGQQME SPDNWLEYGN AWEFPRHNTR YKVRFGGRIQ QEGSKIRWLE TEEILACAYD QIIPGFDTDA TNTLRLWSAQ ASNEINLGKF NQGDYFAAVE DKNHSENVSR VLYPDDSTYS GRELRLRQEY FLVSATVQDI LNRHWAMHHT FNNLADKIAI HLNDTHPVLS IPEMMRLLID EHKFTWMDAW DVVQQVFSYT NHTLMSEALE TWPVDMIGKI LPRHLQIIFD INDHFLKLVE EQYPDDKELL SRVSVIDENN GRRIRMAWLA VIASHKVNGV SALHSELMVQ SLFADFARIF PNRFCNKTNG VTPRRWLGLA NRPLAAVLDD SIGQTWRTDL SQLSELEKNL DYPSFLLALQ KAKLENKKRL AVYIAEKLNI VVNPAALFDV QIKRIHEYKR QLLNVLHVIT RYNRIIDAPD NNWVPRVVIF AGKAASAYYN AKQIIHLIND VAKVINNDPR INNLLKVVFI PNYSVSLAQL IIPAADLSEQ ISLAGTEASG TSNMKFALNG ALTIGTLDGA NVEIREHVGE ENIFIFGNTT EQVEALRKSG YNPRKYYDED PELHQVLTQI ATGTFSPEEP HRYTNLFDSL VNLGDHYQLL ADYRSYVDTQ EQVDALYRNR DEWSRKTLLN IANMGYFSSD RTIKEYADEI WHIKPIRL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGACCTCACCCGTTGTCAGTG  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGCCGGATAGGCTTAAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 12, 2012