Clone

Target

IDP00462  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Nov 02, 2011  

Experiment End Date

Nov 02, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

390  

Reference Sequence Used

MTSRWGKYLL SGIMIAVLAG CQSRPTDRGQ QYKDGRLEQS LELVNEPNAA GKPVNAKDYS DQVKVINQSS PGLYNRNSDT FNAVQNWMLA GADTSKLSLF GLNAYQMEGV DNFGNVQFTG YYTPVLQARY TPQGEFRHPL YRMPAKGKRR LPDRAAIYAG ALDNRNLIIA YTNSLVDNFM MEVQGSGYVD YGDGRPLTFF GYAGKNGHAY RSIGKVLIDR GEVARADMSM QAIRQWAENH SEAEVRELLE QNPSFVFFKP VMYAPVKGAS AVPLIAKASV ASDKSLIPPG TTLLAEVPLL DDQGKFTGKY QMRLMVALDV GGAIKGQHFD IYQGIGHEAG QAAGFYNHYG RVWVLKNAQS SGPLFTAYKG GTQSEPTSND SSLLVNNQDR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGACAAGTCGTTGGGGCAA  

PCR Backward Primer

GGTTCTCCCCACGTCGATCCTGATTATTCACCAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 12, 2012