Clone

Target
IDP00522  
Clone Suffix
4
Experiment Start Date
Nov 02, 2011  
Experiment End Date
Nov 02, 2011  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
opMCSG28  
Sequence Start
1  
Sequence End
183  
Reference Sequence Used
MLLENGWIVG VKQVLSPHFD LRPEGEMPSL LVIHSISLPP SEFGGPYIDQ LFTGTLNADE HPYFASIVHL RVSAHCLIRR DGEIIQYVPF DKRAWHAGVS VFAGRERCND FSIGIELEGT DVLPFTPVQY QRLTEISAVL FAHYPITVER VAGHSEIAPG RKTDPGLAFD WFFYQQNLAK LLV  
Reference Sequence Variance
MATCH  
PCR Forward Primer
GTCTCTCCCATGCTGCTGGAAAACGGCTG  
PCR Backward Primer
GGTTCTCCCCACGTACAAGCAACTTAGCTAAATTTTG  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-11-02 2011-11-02 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay