Clone

Target

IDP01463  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Nov 02, 2011  

Experiment End Date

Nov 02, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

419  

Reference Sequence Used

MEKFRVIGST QPLQGEVTIS GAKNAALPIL FASILAEEPV EVANVPHLRD IDTTMELLER LGAKVERNGS VHVDAGPINQ YCAPYDLVKT MRASIWALGP LVARFGQGQV SLPGGCAIGA RPVDLHIHGL EQLGATITLE DGYVKAHVDG RLQGAHIVMD KVSVGATITI MCAATLAEGT TVLDNAAREP EIVDTAMFLN KLGAKISGAG TDSITIEGVE RLGGGKHAVV PDRIETGTFL VAAAVSRGKI VCRNTHAHLL EAVLAKLEEA GAEIECGEDW ISLDMTGREL KAVTVRTAPH PGFPTDMQAQ FTLLNMMAKG GGVITETIFE NRFMHVPELK RMGAKAEIEG NTVICGDVDR LSGAQVMATD LRASASLVIA GCIAKGETIV DRIYHIDRGY ERIEDKLSAL GANIERFRD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGGAAAAGTTTCGAGTTATTGG  

PCR Backward Primer

GGTTCTCCCCACGGTCGCGGAAACGCTCAATAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 12, 2012