Clone

Target
IDP05459  
Clone Suffix
2
Experiment Start Date
Nov 16, 2011  
Experiment End Date
Nov 16, 2011  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
failed  
Vector
pMCSG7  
Sequence Start
60  
Sequence End
231  
Reference Sequence Used
LEISGEYLLH LSTKLPLYDS NDLKTKTNIE IAEPVVKATK KKNNAYYIQT TSGAGWVNKE NNSLEVQEIH KLSNQKLIVS EETSIYSFPF QSFKEEMKLQ PQTVAPTEQA GNWFKIQMNG TEKWIYAPSA TFEGTKATLF EKKFLWKIEA PHFSKHHLIY LQITFMVLHL KK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGCTTGAAATCTCGGGTGAATATC  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTCTTTAAATGTAACACCATA