Clone

Target
IDP04136  
Clone Suffix
501
Experiment Start Date
Nov 21, 2011  
Experiment End Date
Dec 18, 2011  
Person
Ania Milaczewska  
Lab
University of Virginia  
Protocol ID
uva_cloning_1  
Status
success  
Vector
pet15b with thioredoxin  
Sequence Start
1  
Sequence End
170  
Reference Sequence Used
MGSDKIIHLT DDSFDTDVLK ADGAILVDFW AEWCGPCKMI APILDEIADE YQGKLTVAKL NIDQNPGTAP KYGIRGIPTL LLFKNGEVAA TKVGALSKGQ LKEFLDANLA HMSSHHHHHH SSGRENLYFQ GAAAMAQIRI HEVNTRIENE VKVSKFLQEE GVLYEKWNIS KLPPHLNENY SLTDENKAEI LAVFSKEIAD VSARRGYKAH DVISLSNSTP NLDELLINFQ KEHHHTDDEV RFIVSGHGIF AIEGKDGTFF DVELEPGDLI SVPENARHYF TLQDDRQVVA IRIFVTTEGW VPIY  
PCR Forward Primer
CG GCGGCCGC T ATGGCG CAAATT CGTATT C  
PCR Backward Primer
ATA CTC GAG TTA ATA GAT TGG AAC CCA ACC  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-01-12 2012-01-13 Ania Milaczewska University of Virginia uva_expression_native_1 success E. coli LB IPTG production
2012-01-24 2012-01-25 Ania Milaczewska University of Virginia uva_expression_native_1 success E. coli LB IPTG production
2012-02-13 2012-02-14 Ania Milaczewska University of Virginia uva_expression_semet_1 success E. coli SeMet IPTG production