Clone

Target

IDP00058  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Mar 04, 2013  

Experiment End Date

Mar 04, 2013  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

234  

Reference Sequence Used

MNEIMLETKN LCKTFRQQSV VQNISLSVPK YAIYGLLGPN GAGKSTTLKM ITGMLIPTSG EIYFDGHKWT RHDLHSIGAL IDSPPLYENL TARENLQVRT TVLGLPKSRI DEVLHVVELE NTEKKKAKDF SMGMKQRLGI AIALLNHPKF LILDEPMNGL DPFGIQELRK LILSFPGKGI TVILSSHILS EVSQIADHVG IISGGVLGYQ GKLNKNDDLE DLFVQVAGKY RREA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATGAGATTATGTTAGAAACA  

PCR Backward Primer

TTATCCACTTCCAATGTTATGCCTCCCTTCTATACTTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 21, 2013