Clone

Target

IDP01059  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

256  

Reference Sequence Used

MNDIWWQTYG EGNCHLVLLH GWGLNAEVWH CIREELGSHF TLHLVDLPGY GRSSGFGAMT LEEMTAQVAK NAPDQAIWLG WSLGGLVASQ MALTHPERVQ ALVTVASSPC FSAREGWPGI KPEILGGFQQ QLSDDFQRTV ERFLALQTLG TETARQDART LKSVVLAQPM PDVEVLNGGL EILKTVDLRE ALKNVNMPFL RLYGYLDGLV PRKIVPLLDT LWPHSTSQIM AKAAHAPFIS HPAAFCQALM TLKSSL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATGACATCTGGTGGCAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGCGATGATTTTAGCGTC  

Shipped To Lab

ANL  

Shipped Date

Oct 09, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008