Clone

Target
IDP00271  
Clone Suffix
4
Experiment Start Date
Aug 28, 2012  
Experiment End Date
Aug 28, 2012  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
93  
Sequence End
166  
Reference Sequence Used
VALAPHVGLG LETRAETWMS SEGAWKQIQK VETWALRHPG FTVIALFLAH AIGTSITQKG IIFILLMLVT PSMA  
Reference Sequence Variance
MATCH  
PCR Forward Primer
null  
PCR Backward Primer
null  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-10-10 2012-10-10 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2012-09-07 2012-09-07 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay