Clone

Target

IDP01224  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

638  

Reference Sequence Used

MRVSNVTVRK RLIFILISGI LIFTIIDIRL GYVQFFLGNM LTDRAKDSWS RNITFEPERG KILDRNGVEL ATNKSAPTVF VVPRQIEKPA ETAEKLAAVL GVEKDEIYKR ITKKESIVRL DKGGRKISHD KAKEVRGLSL KGVYIAEDSI RYYPFGNFLS HVLGFAGSDN QGLMGLEKYY DKELNGDEGH VRFFADAKGQ RMPNVGDDFK KPEAGLNLGL TIDARINRIM EREMNIAEST YNPDGMIAIA MNPKNGEILG MSSRPSFDPA DFQSVSPEVY NRNLPVWSTY EPGSTFKIIT LAAALNENLV DLEKDTFYDD GAAEVGGARL KCWKAGGHGS QTFLEVVQNS CNPGFIELGD RLGKERLFKY IRNFGFGQKT GIDLQGEGSG ILFNLDKVGP VEQATTSFGQ GVSVTPIQQV AAVAAAVNGG TLYQPYIAKE FIDPKNNQVV SKKTPVDKRK VISKETSEKV RYALENVVAK GSGKGAFIDG YRVGGKTGTA QKVKDGKYLE NNYIVSFIGF APADDPEIVV YVAVDNPKGV TQFGGVVAAP IVGNILRDAL PVMGVKPRKE QVEREYKWGD IPTVEVPNLI GMKKKDLQTQ LVDLKLDISG DGEKVIKQSP EAGAKVKEGS KIRIYFGN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCGTGTATCAAATGTAACAG  

PCR Backward Primer

TTATCCACTTCCAATGTTAATTCCCGAAGTAAATTCTAAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008